Differently colored complexity factor values are presented. These values were divided into three broad ranges: a red 0.55. b Blue elements represent rounded single cells and yellow elements the more elongated structures. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/14960617 c Blue and yellow elements represent rounded cells and elongated structures, respectively, combined with more red elements, representing long and complex vessels. d Dominance of red elements representing long and complex vessels and less yellow and blue elements for rounded shapesFreiman et al. Stem Cell Research Therapy (2016) 7:Page 8 ofcell combinations (60 vs. 40 , p n-Phenylpiperazine-1-carboxamide 90 of the HAMEC:MSC-embedded scaffold elements exhibited elongated shapes, while complexity values equal to those recorded on day 10 were measured for the HUVEC:MSC and HAMEC:HNDF groups on day 14 (p PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9544797 addition, the HUVEC:MSC group showed the lowest relative -SMA expression on all tested days (Fig. 7b) when compared to other cell combinations.-SMA expression as an indicator for vessel maturityFig. 5 Distribution of complexity factor ranges for the different cell pairs. The complexity factor of networks within cell-embedded scaffolds was determined on days 4, 7, 10 4,4,5,5-Tetramethyl-2-(2-methylprop-1-en-1-yl)-1,3,2-dioxaborolane and 14 post-seeding. The distribution of complexity factors per cell pair and per day of analysis is presented. a Day 4 (p s12905-016-0352-0 HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cellFreiman et al. Stem Cell Research Therapy (2016) 7:Page 9 ofFig. 6 Vessel alignment as an indication of vessel maturity. a Representative image of half of a scaffold showing different regions with orientated vessel.